4L1U

Crystal Structure of Human Rtf1 Plus3 Domain in Complex with Spt5 CTR Phosphopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
Unit cell lengths	112.932, 172.514, 58.476
Unit cell angles	90.00, 107.00, 90.00

Refinement procedure

Resolution	46.923 - 2.424
R-factor	0.1801
Rwork	0.178
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l1p
RMSD bond length	0.009
RMSD bond angle	1.116
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.0)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.520
High resolution limit [Å]	2.424	5.230	2.424
Rmerge	0.068	0.059	0.376
Number of reflections	40281
<I/σ(I)>	8.8
Completeness [%]	99.9	99.9	99.9
Redundancy	3.6	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	Sodium acetate, Ammonium sulfate, pH 4.2, vapor diffusion, sitting drop, temperature 293K