4L1M

Structure of the first RCC1-like domain of HERC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97926
Spacegroup name	P 32 2 1
Unit cell lengths	94.159, 94.159, 281.894
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.600
R-factor	0.201
Rwork	0.199
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kci
RMSD bond length	0.011
RMSD bond angle	1.289
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.965	46.982	2.740
High resolution limit [Å]	2.600	8.220	2.600
Rmerge		0.011	0.011
Total number of observations		15451	72854
Number of reflections	45698
<I/σ(I)>	13.8	12.5	0.7
Completeness [%]	100.0	99.4	100
Redundancy	10.7	9.5	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	291	1.5 M ammonium sulfate, 0.1 M Tris-hydrochloride, pH 8.5, vapor diffusion, temperature 291K