4L1L
Rat PKC C2 domain bound to CD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-03-25 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 58.224, 58.224, 88.539 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.816 - 1.600 |
| R-factor | 0.16 |
| Rwork | 0.157 |
| R-free | 0.19530 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.225 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.270 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 43697 | |
| <I/σ(I)> | 22.4 | 7.2 |
| Completeness [%] | 98.6 | 94.3 |
| Redundancy | 20 | 17.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 298 | 0.25 M Lithium Sulfate, 20% PEG 3350, pH 5.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
