4L0O
Structure determination of cystathionine gamma-synthase from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 115.812, 174.890, 324.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.760 |
| R-factor | 0.23626 |
| Rwork | 0.234 |
| R-free | 0.27793 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3E6G; Cystathionine C-Lyase-Like Protein From Xanthomonas Oryzae Pv.Oryzae |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.335 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.810 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 80506 | |
| <I/σ(I)> | 28.16 | 3.5 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 7.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 289 | 20%MPD, 20%Peg1000, 20%Peg3350, 0.03M Halogen mixture, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
