4L0J
Structure of a translocation signal domain mediating conjugative transfer by type IV secretion systems
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.8726 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 109.173, 109.173, 56.801 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.820 - 1.850 |
R-factor | 0.1807 |
Rwork | 0.178 |
R-free | 0.22789 |
Structure solution method | SAD |
RMSD bond length | 0.023 |
RMSD bond angle | 2.253 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.820 | 2.170 | 1.910 |
High resolution limit [Å] | 1.850 | 2.110 | 1.850 |
Number of reflections | 29933 | ||
Completeness [%] | 100.0 | 100 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.1 M Bis-Tris propane, pH 6.5, 0.2 M lithium sulfate, 25% w/v PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 290K |