4KZG
Crystal structure of zebrafish MO25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-09-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 156.665, 156.665, 221.251 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.956 - 2.900 |
| R-factor | 0.2889 |
| Rwork | 0.288 |
| R-free | 0.32660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1upl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.881 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.172 | 0.467 |
| Number of reflections | 70049 | |
| <I/σ(I)> | 3.86 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2M Lithium citrate tribasic tetrahydrate, 20% w/v Polyethylene glycol 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
