4KYW
Restriction endonuclease DPNI in complex with two DNA molecules
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.23953 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.700, 101.050, 114.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.230 - 2.350 |
| R-factor | 0.20216 |
| Rwork | 0.201 |
| R-free | 0.21904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4esj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.340 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.049 | 0.440 |
| Number of reflections | 23502 | |
| <I/σ(I)> | 20.51 | 4.14 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 6.37 | 6.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 160 mM CALCIUM ACETATE, 80 mM SODIUM CACODYLATE PH 6.5, 14.4% W/V PEG 8000, 20% V/V GLYCEROL, 10 mM SPERMIDINE., VAPOR DIFFUSION, SITTING DROP, temperature 277K |
