4KYI
Crystal structure of the phospholipase VipD from Legionella pneumophila in complex with the human GTPase Rab5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 |
| Unit cell lengths | 94.275, 97.976, 109.849 |
| Unit cell angles | 76.57, 80.71, 78.91 |
Refinement procedure
| Resolution | 29.827 - 3.075 |
| R-factor | 0.2353 |
| Rwork | 0.233 |
| R-free | 0.28030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.631 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.260 | |
| High resolution limit [Å] | 3.070 | 8.860 | 3.070 |
| Rmerge | 0.060 | 0.014 | 0.631 |
| Number of reflections | 67490 | 2720 | 10270 |
| <I/σ(I)> | 17.01 | 72.19 | 2.09 |
| Completeness [%] | 97.4 | 93.7 | 92.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 16% PEG6000, 0.1 M Tris-HCl, 0.2 M LiCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
