4KXH

The X-ray crystal structure of a dimeric variant of human pancreatic ribonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-11
Detector	MAR CCD 165 mm
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	71.506, 74.319, 128.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.820 - 2.700
R-factor	0.20074
Rwork	0.198
R-free	0.24587
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.675
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CCP4 (suite (iMosflm))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.700
Number of reflections	19485
Completeness [%]	100.0
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% PEG 8000, 0.1M ammonium sulphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K