4KWX

Linear structure of the Holliday junction sequence (TCGGCGCCGA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97630
Spacegroup name	P 1
Unit cell lengths	34.410, 34.490, 34.690
Unit cell angles	101.28, 103.21, 103.81

Refinement procedure

Resolution	32.299 - 1.300
R-factor	0.1737
Rwork	0.173
R-free	0.18700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.263
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.570	32.570	1.340
High resolution limit [Å]	1.300	5.370	1.300
Rmerge	0.030	0.030	0.280
Number of reflections	31546
<I/σ(I)>		18.61	2.31
Completeness [%]	87.9	93.99	87.88
Redundancy	1.77	1.83	1.74

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	285	0.75-1.5 mM DNA, 40-60% MPD, magnesium chloride, potassium chloride, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K