4KWE
GDP-bound, double-stranded, curved FtsZ protofilament structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 200 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 132.509, 132.509, 321.549 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.950 - 2.910 |
| R-factor | 0.24204 |
| Rwork | 0.241 |
| R-free | 0.26749 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.981 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.134 | 0.634 |
| Number of reflections | 37414 | |
| <I/σ(I)> | 65.7 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 21.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 3M Na formate, 0.1M Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
