4KVT

Crystal structure of a 6-helix coiled coil CC-Hex-L24C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	65
Detector technology	PIXEL
Collection date	2012-10-06
Detector	PSI PILATUS 6M
Wavelength(s)	0.92
Spacegroup name	P 42 21 2
Unit cell lengths	54.430, 54.430, 147.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.098 - 1.600
R-factor	0.1923
Rwork	0.191
R-free	0.20840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.912
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	34.160
High resolution limit [Å]	1.600
Number of reflections	29509
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	0.1 M Tris, 0.1 M potassium chloride with 15 % w/v PEG 2000 MME, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K