4KVO
The NatA (Naa10p/Naa15p) amino-terminal acetyltrasferase complex bound to AcCoA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-02 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.739, 119.692, 132.024 |
| Unit cell angles | 80.28, 76.85, 70.65 |
Refinement procedure
| Resolution | 49.047 - 3.150 |
| R-factor | 0.2184 |
| Rwork | 0.217 |
| R-free | 0.24600 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.790 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.076 | 0.602 |
| Number of reflections | 77244 | |
| <I/σ(I)> | 17.2 | 2.4 |
| Completeness [%] | 99.0 | 98.4 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Protein Buffer: 25 mM Hepes, 200 mM NaCl, 1 mM TCEP Crystallization well: 100 mM Hepes, 10% Peg 3350, 10% isopropanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
