4KU4
Crystal Structure of a Ras-like Protein from Cryphonectria parasitica in Complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.795, 52.595, 53.234 |
| Unit cell angles | 114.62, 93.01, 90.05 |
Refinement procedure
| Resolution | 44.500 - 1.600 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ce2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.510 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.075 | 0.313 |
| Number of reflections | 42642 | |
| <I/σ(I)> | 11.07 | 2.9 |
| Completeness [%] | 88.4 | 62.8 |
| Redundancy | 3.5 | 2.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 5 mM GDP, 0.2 M Magnesium Format, 0.1 M HEPES, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
