4KTU
Bovine trypsin in complex with microviridin J at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 71.230, 71.230, 72.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.350 |
| R-factor | 0.13617 |
| Rwork | 0.135 |
| R-free | 0.16044 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mwy |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.915 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.051 | 0.351 |
| Number of reflections | 47008 | |
| <I/σ(I)> | 17 | 4.1 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M sodium cacodylate, 0.2 M ammonium sulfate, 30% PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
