4KTT

Structural insights of MAT enzymes: MATa2b complexed with SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-20
Detector	PSI PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	72.442, 115.723, 298.455
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.410 - 2.590
R-factor	0.221
Rwork	0.218
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2PO2 AND 2YDY
RMSD bond length	0.006
RMSD bond angle	1.091
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.590	2.600
Rmerge	0.144
Number of reflections	77361
Completeness [%]	98.7	90.1
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	298	0.1M MES/Imidazole pH 6.5 0.1M Carboxylic acids 20% Ethylene glycol 10% PEG 8K, VAPOR DIFFUSION, HANGING DROP, temperature 298K