4KT8

The complex structure of Rv3378c-Y51FY90F with substrate, TPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	106.287, 106.287, 66.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.400
Rwork	0.220
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3VX5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.065	0.397
Number of reflections	15419
<I/σ(I)>		6.4
Completeness [%]	99.7	100
Redundancy	10.3	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1