4KSJ
Crystal structure of the OTU domain of Gumby/Fam105B at 1.6 angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97919 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.350, 71.820, 94.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.376 - 1.600 |
R-factor | 0.18215 |
Rwork | 0.180 |
R-free | 0.21547 |
Structure solution method | SAD |
RMSD bond length | 0.030 |
RMSD bond angle | 2.201 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.376 | 1.720 | 1.660 |
High resolution limit [Å] | 1.600 | 1.660 | 1.600 |
Rmerge | 0.061 | 0.416 | 0.584 |
Number of reflections | 36961 | ||
<I/σ(I)> | 36.6 | 4.5 | 3.1 |
Completeness [%] | 99.9 | 100 | 100 |
Redundancy | 3.8 | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 100mM MES, 200mM MgCl2, 19% PEG3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP |