4KRO
Nanobody/VHH domain EgA1 in complex with the extracellular region of EGFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.207, 96.288, 128.097 |
| Unit cell angles | 90.00, 100.68, 90.00 |
Refinement procedure
| Resolution | 38.193 - 3.054 |
| R-factor | 0.2235 |
| Rwork | 0.221 |
| R-free | 0.27950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1YY9 AND 4KRN |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.897 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.100 |
| High resolution limit [Å] | 3.050 | 8.270 | 3.050 |
| Rmerge | 0.099 | 0.030 | 0.463 |
| Number of reflections | 29807 | ||
| <I/σ(I)> | 10.2 | ||
| Completeness [%] | 98.8 | 92.3 | 99.6 |
| Redundancy | 2.3 | 2.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 17.5% PEG3350, 1.5 M sodium chloride, 5% glycerol, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
