4KRF
Structure of Human Argonaute-1 let-7 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.075 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.390, 97.730, 72.700 |
Unit cell angles | 90.00, 110.89, 90.00 |
Refinement procedure
Resolution | 40.568 - 2.101 |
R-factor | 0.1772 |
Rwork | 0.175 |
R-free | 0.21730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.723 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.160 | |
High resolution limit [Å] | 2.100 | 9.400 | 2.100 |
Rmerge | 0.041 | 0.022 | 0.320 |
Number of reflections | 50618 | 625 | 2558 |
<I/σ(I)> | 16.85 | 44.3 | 2.43 |
Completeness [%] | 94.2 | 97.5 | 64.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 13% PEG 3350, 9% iso-propanol, 0.1M Tris pH 9.0, vapor diffusion, hanging drop, temperature 291K |