4KQV
Topoisomerase iv atp binding domain of francisella tularensis in complex with a small molecule inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-21 |
| Detector | NOIR-1 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.200, 163.160, 72.280 |
| Unit cell angles | 90.00, 91.81, 90.00 |
Refinement procedure
| Resolution | 66.060 - 2.380 |
| R-factor | 0.25727 |
| Rwork | 0.255 |
| R-free | 0.29851 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hy1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.373 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 66.060 |
| High resolution limit [Å] | 2.370 |
| Rmerge | 0.076 |
| Number of reflections | 40074 |
| Completeness [%] | 96.0 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.4 | 277 | 10% PEG 4000, 10% ISOPROPANOL, 100 MM CITRATE, pH 5.4, VAPOR DIFFUSION, temperature 277K |
