4KQP
Crystal structure of Lactococcus lactis GlnP substrate binding domain 2 (SBD2) in complex with glutamine at 0.95 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.990, 51.690, 44.230 |
Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
Resolution | 44.200 - 0.950 |
R-factor | 0.1149 |
Rwork | 0.115 |
R-free | 0.11970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.281 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.200 | 1.000 |
High resolution limit [Å] | 0.950 | 0.950 |
Rmerge | 0.042 | 0.380 |
Number of reflections | 119821 | |
<I/σ(I)> | 19.8 | 3.24 |
Completeness [%] | 98.5 | 95.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 281 | 30% PEG 6000, 100MM CACL2, 0.1M SODIUM ACETATE BUFFER, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |