4KQE

The mutant structure of the human glycyl-tRNA synthetase E71G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Detector technology	CCD
Collection date	2011-11-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97916
Spacegroup name	P 43 21 2
Unit cell lengths	91.239, 91.239, 246.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.665 - 2.739
R-factor	0.2125
Rwork	0.210
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pme
RMSD bond length	0.003
RMSD bond angle	0.785
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.739	2.750
Rmerge	0.170	0.966
Number of reflections	28122
<I/σ(I)>	11.7	2.5
Completeness [%]	99.3	99
Redundancy	7.1	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	0.1M ammonium citrate, 17% PEG3350, 0.1M Tris-HCl, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K