Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Detector technology | CCD |
| Collection date | 2011-06-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.750, 84.840, 310.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.430 - 2.240 |
| R-factor | 0.20137 |
| Rwork | 0.200 |
| R-free | 0.25236 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ejb |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.002 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.430 | 2.360 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.083 | 0.280 |
| Number of reflections | 105615 | |
| <I/σ(I)> | 15 | 5.8 |
| Redundancy | 5.31 | 2.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 1.6 M AMMONIUM SULPHATE, 100 mM SODIUM, ACETATE AND 100 mM SODIUM CHLORIDE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
