4KPQ
Structure and receptor binding specificity of the hemagglutinin H13 from avian influenza A virus H13N6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-16 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.106, 77.061, 76.474 |
| Unit cell angles | 85.50, 82.55, 87.37 |
Refinement procedure
| Resolution | 42.353 - 2.502 |
| R-factor | 0.2255 |
| Rwork | 0.224 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f23 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.736 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 57001 | |
| Completeness [%] | 98.0 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.2M L-Proline, 0.1M HEPES, 10% PEG-3350, 0.01M Sodium Bromide, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
