4KPI
Rotational order-disorder structure of reversibly photoswitchable red fluorescent protein rsTagRFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 92.697, 92.697, 53.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.313 - 1.580 |
| R-factor | 0.2274 |
| Rwork | 0.225 |
| R-free | 0.27880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u8a |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.789 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_1090) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 3.400 | 1.580 |
| Rmerge | 0.046 | 0.029 | 0.596 |
| Number of reflections | 15284 | ||
| <I/σ(I)> | 15.9 | ||
| Completeness [%] | 94.4 | 99.3 | 62.2 |
| Redundancy | 6.8 | 6.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M ammonium nitrate 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
