4KPG

Crystal structure of MycP1 from the ESX-1 type VII secretion system

Experimental procedure

Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Detector technology	CCD
Collection date	2011-08-15
Detector	RAYONIX MX-300
Wavelength(s)	0.97895
Spacegroup name	I 2 2 2
Unit cell lengths	81.000, 94.080, 119.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.040 - 2.148
R-factor	0.1676
Rwork	0.166
R-free	0.19670
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.049
Data scaling software	SCALA (CCP4_3.3.16)
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall
Low resolution limit [Å]	59.930
High resolution limit [Å]	2.148
Number of reflections	25383
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M NaF, 25% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K