4KNH

Structure of the Chaetomium thermophilum adaptor nucleoporin Nup192 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-03
Detector	PSI PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	102.874, 102.874, 443.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.766 - 2.702
R-factor	0.1935
Rwork	0.192
R-free	0.23120
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.569
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	66696
<I/σ(I)>	21.6	3.1
Completeness [%]	100.0	100
Redundancy	12.9	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	294	6% PEG4000, 0.1 M MES, pH 5.7, 0.6 M magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 294K