4KMZ

Human folate receptor beta (FOLR2) in complex with the folate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-24
Detector	NOIR-1
Wavelength(s)	1.0000
Spacegroup name	P 61 2 2
Unit cell lengths	96.861, 96.861, 98.337
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.430 - 2.300
R-factor	0.2108
Rwork	0.205
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kmx
RMSD bond length	0.006
RMSD bond angle	0.930
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.089	0.034	0.489
Number of reflections	12052
<I/σ(I)>	7.9
Completeness [%]	95.2	95.5	85.5
Redundancy	11.1	10.6	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	2.4 M Ammonium phosphate diabasic, 0.1 M Hepes pH 7.5, Vapor diffusion, sitting drop, temperature 293K