4KMU

X-ray crystal structure of the Escherichia coli RNA polymerase in complex with Rifampin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-12
Detector	ADSC QUANTUM 210
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	184.520, 203.873, 307.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.966 - 3.850
R-factor	0.2667
Rwork	0.264
R-free	0.32080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4IGC
RMSD bond length	0.002
RMSD bond angle	0.649
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8.2_1309)
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.810
High resolution limit [Å]	3.750	3.750
Number of reflections	103271
<I/σ(I)>	5.6	1.4
Completeness [%]	90.6	59.3
Redundancy	4.2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	300	0.1 M HEPES-HCl, pH 7.0, 0.2 M calcium acetate, ~15% PEG400, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 300K