4KMO

Crystal Structure of the Vps33-Vps16 HOPS subcomplex from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	100.274, 100.274, 176.225
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.656 - 2.600
R-factor	0.2267
Rwork	0.225
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jc8
RMSD bond length	0.002
RMSD bond angle	0.634
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.111	0.079	0.873
Number of reflections	31686
<I/σ(I)>	6.4
Completeness [%]	98.1	96.5	94
Redundancy	9.3	9.1	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	12-16% (w/v) PEG 4000, 150 mM ammonium sulfate, 100 mM MES buffer , pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K