4KML
Probing the N-terminal beta-sheet conversion in the crystal structure of the full-length human prion protein bound to a Nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-04-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.442, 45.921, 44.964 |
| Unit cell angles | 90.00, 96.09, 90.00 |
Refinement procedure
| Resolution | 31.610 - 1.500 |
| R-factor | 0.17608 |
| Rwork | 0.175 |
| R-free | 0.19211 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w9e |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.299 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 42289 | |
| Completeness [%] | 96.9 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | A9 of the MD-proplex screen - 0.2 M sodium chloride, 0.1 M MES pH 6.0, 20% w/v PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
