4KM9
Crystal structure of human Suppressor of Fused
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-25 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.875, 120.940, 117.653 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.190 |
| R-factor | 0.2462 |
| Rwork | 0.243 |
| R-free | 0.30530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4km8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.970 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 63.040 | 50.000 | 3.310 |
| High resolution limit [Å] | 3.190 | 6.890 | 3.200 |
| Rmerge | 0.127 | 0.103 | 0.492 |
| Number of reflections | 8956 | ||
| <I/σ(I)> | 16.2 | ||
| Completeness [%] | 99.1 | 94.9 | 100 |
| Redundancy | 8.4 | 7.2 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | 20% PEG 3350, 0.2M Potassium chloride, vapor diffusion, hanging drop, temperature 281K |
