4KM6

Human folate receptor alpha (FOLR1) at acidic pH, orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-02
Detector	NOIR-1
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	36.519, 63.339, 71.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.491 - 1.550
R-factor	0.171
Rwork	0.168
R-free	0.19990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.163
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.087	0.038	0.495
Number of reflections	24336
<I/σ(I)>	10
Completeness [%]	98.3	98.6	86.8
Redundancy	5.7	5.6	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	12 mg/mL co-purified with methotrexate in 0.1 M sodium citrate, , 35 % (v/v) jeffamine ED-2001 (MTX not present in structure), pH 5.5, Vapor diffusion, sitting drop, temperature 293K