4KLR
E343Q variant of human ferrochelatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | CCD |
| Collection date | 2010-09-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.127, 92.561, 109.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.710 - 2.180 |
| R-factor | 0.20278 |
| Rwork | 0.200 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.213 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 70.710 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 49841 |
| Completeness [%] | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | Crystals were grown using Hanging drop with a precipitating solution consisting of 0.1 Bis-Tris ph 6.5, 0.075 M Ammonium sulfate and 30 % pentaerythritol ethoxylate (15/4 EO/OH), VAPOR DIFFUSION, HANGING DROP |
