4KLH
DNA polymerase beta matched product complex with Mn2+, 40 s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-10 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.900, 80.200, 55.100 |
Unit cell angles | 90.00, 107.90, 90.00 |
Refinement procedure
Resolution | 25.089 - 1.876 |
R-factor | 0.2109 |
Rwork | 0.205 |
R-free | 0.26940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
RMSD bond length | 0.012 |
RMSD bond angle | 1.114 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 | 1.950 |
High resolution limit [Å] | 1.876 | 2.120 | 1.876 |
Rmerge | 0.075 | 0.217 | 0.516 |
Number of reflections | 33530 | ||
<I/σ(I)> | 19.4 | 5.18 | 2.039 |
Completeness [%] | 97.3 | 98.6 | 88.6 |
Redundancy | 4.6 | 3.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |