4KL1
HCN4 CNBD in complex with cGMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-01-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97240 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.100, 99.030, 109.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.620 - 2.700 |
R-factor | 0.21403 |
Rwork | 0.210 |
R-free | 0.27226 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u11 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.862 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.620 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.100 | 0.450 |
Number of reflections | 29735 | |
<I/σ(I)> | 18.1 | 4.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 10-15% PEG3350, 200 mM sodium acetate buffer, 200 mM ammonium phosphate, 5-50mM cGMP, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |