4KKR

Crystal structure of Vibrio cholerae RbmA (crystal form 3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-07
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9771
Spacegroup name	P 41 21 2
Unit cell lengths	119.404, 119.404, 104.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.284 - 2.000
R-factor	0.1619
Rwork	0.161
R-free	0.19160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.042
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	51215
Completeness [%]	99.9	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	294	0.1 M BIS-TRIS, 3.0 M NaCl, 25% Xylitol, 0.16% Thiamine monophosphate chlroide dihydrate, 0.16% Acetylsalicylic acid, 0.16% Cholic acid, 0.16% 1,2,3-Heptanetriol, 0.16% Vanillin, 0.16% N-Acetly-D-mannosamine, 0.02 M HEPES sodium, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K