4KK5

Structure of the CLC-ec1 deltaNC construct in 20mM fluoride and 20mM bromide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.919
Spacegroup name	C 1 2 1
Unit cell lengths	229.127, 98.166, 169.761
Unit cell angles	90.00, 131.73, 90.00

Refinement procedure

Resolution	39.782 - 3.171
R-factor	0.2339
Rwork	0.232
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.814
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.170
Number of reflections	91572
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	295	100mM Na/K tartrate, 26%PEG400, 100mM Glycine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K