4KK0

Crystal Structure of TSC1 core domain from S. pombe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	231.375, 239.791, 112.014
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.740 - 2.900
R-factor	0.19526
Rwork	0.192
R-free	0.25646
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.639
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.900
Number of reflections	140033
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	75mM 3Na Citrate, 25mM HEPES, 500mM NaCl, 1.5% PEI, 6.25% 2-propanol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K