4KJZ

Crystal Structure of Thermus Thermophilus IF2, Apo and GDP-bound Forms (2-474)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97923
Spacegroup name	P 1 21 1
Unit cell lengths	119.830, 177.816, 61.793
Unit cell angles	90.00, 89.87, 90.00

Refinement procedure

Resolution	46.760 - 2.800
R-factor	0.24138
Rwork	0.240
R-free	0.27564
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b3x
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.010	2.900
High resolution limit [Å]	2.800	3.530	2.800
Number of reflections	63086
Completeness [%]	100.0	100	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	292	40 mM sodium cacodylate, pH 5.4, 100 mM calcium acetate, 10 mM glycyl-glycine, 10 mM taurine, 8% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 292K