4KJG
Structure of Rat Intestinal Alkaline Phosphatase expressed in insect cell
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-14 |
| Detector | RAYONIX MX300HS |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.306, 167.170, 71.843 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.420 - 2.380 |
| R-factor | 0.1663 |
| Rwork | 0.164 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.470 |
| High resolution limit [Å] | 2.380 | 5.130 | 2.380 |
| Rmerge | 0.171 | 0.079 | 0.574 |
| Number of reflections | 44083 | ||
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 99.9 | 99.9 | 100 |
| Redundancy | 5.6 | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 1.4 M Sodium citrate, 0.1 M Hepes, pH 7.5, Vapor diffusion, temperature 293K |
