4KIT

Crystal structure of human Brr2 in complex with the Prp8 Jab1/MPN domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-19
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 42 21 2
Unit cell lengths	240.002, 240.002, 201.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.204 - 3.598
R-factor	0.1991
Rwork	0.197
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4F91 AND 2OG4
RMSD bond length	0.008
RMSD bond angle	1.211
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.800
High resolution limit [Å]	3.598	3.598
Rmerge	0.170	0.725
Number of reflections	68276
<I/σ(I)>	14.4	2.4
Completeness [%]	99.8	99.2
Redundancy	7.5	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	277	0.1 M HEPES-NaOH, pH 7.9, 0.2 M magnesium chloride, 12% ethanol, Silver Bullets Bio condition 91 (Hampton Research) as additive, VAPOR DIFFUSION, HANGING DROP, temperature 277K