4KI5

Cystal structure of human factor VIII C2 domain in a ternary complex with murine inhbitory antibodies 3E6 and G99

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-07-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54187
Spacegroup name	P 2 21 21
Unit cell lengths	54.250, 71.160, 277.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.428 - 2.470
R-factor	0.1903
Rwork	0.188
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.428	56.428	2.610
High resolution limit [Å]	2.470	7.820	2.470
Rmerge		0.066	0.840
Total number of observations		17885	78388
Number of reflections	39550
<I/σ(I)>		8.5	0.9
Completeness [%]	99.9	99.7	99.7
Redundancy	14	12.6	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	28% PEG-1000, 0.15 M sodium acetate, 0.025 M Tris-HCl, 0.05 M NaCl, 0.15 M ammonium nitrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K