4KHN
Crystal structure of the ternary complex of the D714A mutant of RB69 DNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.258, 119.353, 148.031 |
| Unit cell angles | 90.00, 91.64, 90.00 |
Refinement procedure
| Resolution | 35.334 - 2.550 |
| R-factor | 0.1704 |
| Rwork | 0.168 |
| R-free | 0.21090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.192 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1363)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 2.559 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.063 | 0.617 |
| Number of reflections | 84020 | |
| <I/σ(I)> | 16.8 | 2.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 12% PEG 8000, 0.1 M MES, 0.2 Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6 |
