4KH8

Crystal structure of a Lipocalin-like protein (EF0376) from Enterococcus faecalis V583 at 1.60 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91837,0.97903,0.97871
Spacegroup name	P 65
Unit cell lengths	99.745, 99.745, 56.956
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.046 - 1.600
R-factor	0.1424
Rwork	0.141
R-free	0.17270
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.533
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.046	27.046	1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge		0.073	0.681
Total number of observations		2934	20074
Number of reflections	42670
<I/σ(I)>	10.5	7.9	1.1
Completeness [%]	99.9	98.2	100
Redundancy	6.2	5.9	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	12.0% Glycerol, 1.5M ammonium sulfate, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K