4KGG
Crystal structure of light mutant2 and dcr3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 148.754, 148.754, 148.754 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.630 - 2.780 |
| R-factor | 0.18087 |
| Rwork | 0.178 |
| R-free | 0.23466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j6g |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.983 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.830 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.140 | 0.691 |
| Number of reflections | 27912 | |
| <I/σ(I)> | 20.1429 | 4.12 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.5 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 290 | 0.2 M Magnesium Chloride, 0.1 M Tris:HCl, 2.5 M Sodium Chloride, PH7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
