4KFW
Structural insight into Golgi membrane stacking by GRASP65 and GRASP55
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Detector technology | CCD |
Collection date | 2012-07-30 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 83.077, 83.077, 145.153 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.810 - 2.700 |
R-factor | 0.2067 |
Rwork | 0.202 |
R-free | 0.28990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.112 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.100 |
High resolution limit [Å] | 2.700 | 6.890 | 2.700 |
Number of reflections | 14443 | ||
Completeness [%] | 98.2 | 98.1 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 8.5 | 293 | 0.1M Tris (pH 8.5) 25% PEG3350 0.2 M (NH4)2Ac, LIQUID DIFFUSION, temperature 293K |