4KFE
Crystal structure of Hansenula polymorpha copper amine oxidase-1 reduced by methylamine at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.577, 222.836, 103.619 |
| Unit cell angles | 90.00, 95.87, 90.00 |
Refinement procedure
| Resolution | 49.030 - 2.100 |
| R-factor | 0.13155 |
| Rwork | 0.129 |
| R-free | 0.18033 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.889 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 | 0.410 |
| Number of reflections | 265779 | |
| <I/σ(I)> | 12.3 | 1.83 |
| Completeness [%] | 98.6 | 89.4 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 8.0-9.5% PEG8000, 0.28-0.30 M phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
