4KFD
Crystal structure of Hansenula polymorpha copper amine oxidase-1 reduced by methylamine at pH 6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-16 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.577, 222.836, 103.619 |
| Unit cell angles | 90.00, 95.87, 90.00 |
Refinement procedure
| Resolution | 46.780 - 1.690 |
| R-factor | 0.136 |
| Rwork | 0.134 |
| R-free | 0.16400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.996 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.780 | |
| High resolution limit [Å] | 1.690 | 1.700 |
| Rmerge | 0.090 | 0.590 |
| Number of reflections | 513107 | |
| <I/σ(I)> | 14.1 | 1.7 |
| Completeness [%] | 99.5 | 97.3 |
| Redundancy | 4.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 298 | 8.0-9.5% PEG8000, 0.28-0.30 M phosphate , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
